Journal: PLoS ONE

Article Title: Mesenchymal Transition and PDGFRA Amplification/Mutation Are Key Distinct Oncogenic Events in Pediatric Diffuse Intrinsic Pontine Gliomas

doi: 10.1371/journal.pone.0030313

Figure Lengend Snippet: Panel A: Radial plot showing the expression of oligodendroglial markers in the two groups of DIPG, in log2 ratios related each other. The red circle represent the median expression level of the whole population of DIPG. Group 1 expresses higher levels of oligodendroglial markers than group 2 DIPG. Panel B: Morphological oligodendroglial differenciation in group 1 tumors (HES staining, ×40). Panel C: Morphological astrocytic differenciation in group 2 tumors (HES staining ×40). Panel D: Olig2 immunohistochemistry in a group 1 DIPG showing that probably not all cells in the biopsy are tumoral (×40). Panel E: Dual immunohistochemistry for Olig2 and GFAP showing that tumor cells in mitosis are GFAP negative but Olig2 positive (×100). Panel F: Overall survival of 55 DIPG according to the presence (red) or absence (blue) of oligodendroglial differenciation. Median OS was shorter in patients with oligodendroglial type of tumors (7.73 vs 12.37, p = 0.045, log rank test).

Article Snippet: Sections were then incubated with various commercial monoclonal primary antibodies against Olig2 (AF 2418, 1/150, R/D system, CA, USA), P53 (DO-1, 1/1, Ventana) and MIB-1 (1/100; Dako, Glostrup, Denmark).

Techniques: Expressing, Staining, Immunohistochemistry

Journal: STAR Protocols

Article Title: Utilizing mouse optic nerve crush to examine CNS remyelination

doi: 10.1016/j.xpro.2021.100796

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Olig2 (1:300) , Novus biologicals , NBP1-28667.

Techniques: Transduction, Virus, Recombinant, Sterility, Saline, Imaging, Positive Control, Software, Microscopy

Journal: iScience

Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

doi: 10.1016/j.isci.2026.114718

Figure Lengend Snippet: Aurkb deficiency in microglia disrupts remyelination and oligodendrocyte density in the CPZ-induced demyelination model (A) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl mice (2–3 months old) were i.p. injected with TAM for 5 consecutive days. After one month, the mice were fed a 0.25% CPZ-enriched diet for 5 weeks to induce demyelination (CPZ group) or followed by a CPZ-free normal diet for an additional 2 weeks (Recovery group). The corpus callosum (CC) was analyzed by immunofluorescence (IF) and Black-Gold II myelin staining. (B and C) Representative Black-Gold II myelin staining images and quantification of myelin intensity ( n = 5 mice per genotype per group, Scale bars: 200 μm). (D–F) Representative immunofluorescence analysis and quantification of total Mbp and oligodendrocytes (Olig2 + ), and g-h) representative immunofluorescence analysis and quantification of mature oligodendrocytes (CC-1 + ) ( n = 5 mice per genotype per group, Scale bars: 50 μm). Data are presented as the mean ± SD. two-way ANOVA with Bonferroni multiple comparisons test in (C, E, F, and H); ∗∗ p < 0.01 and ∗∗∗ p < 0.001; ns, not significant compared with the Aurkb fl/fl group.

Article Snippet: Rabbit anti-Olig2 Antibody , Proteintech , Cat# 13999-1-AP, RRID: AB_2157541.

Techniques: Injection, Immunofluorescence, Staining